Plasma Soluble Fms-like Tyrosine Kinase-1 Works as Sole Predictor of Pre-Eclampsia at Mid-Trimester of Pregnancy

Khalid Yousuf Memon; Ikramdin Ujjan; Nailla Yousuf Memon; Syed Hasan Ala; Fozia shaik; Abid Hussain Chang

Khalid Yousuf Memon Department of Pathology, Liaquat University of Medical & Health Sciences, Jamshoro
Ikramdin Ujjan Department of Pathology, Liaquat University of Medical & Health Sciences, Jamshoro
Nailla Yousuf Memon Department of Gynecology/Obstetrics, Unit 1 Peoples University of Medical & Health Sciences, Banzirabad
Syed Hasan Ala Department of Gynecology/Obstetrics, Dow University Of Medical & Health Sciences, Karachi.
Fozia shaik Department of biochemistry, Liaquat University of Medical & Health Sciences, Jamshoro.
Abid Hussain Chang Department of Pathology, Liaquat University of Medical & Health Sciences, Jamshoro.

Keywords: Soluble fms-like tyrosine kinase-1

Abstract

Objective: To determine the soluble fms-like tyrosine kinase-1 (sFlt-1) as a predictor of pre-eclampsia at mid trimester of pregnancy.

Methodology: A Case-Control study was carried out in the departments of Obstetrics/Gynecology and Pathology, at LUMHS, Hospital Jamshoro/Hyderabad over a period of twelve months from 06-02-2016 to 06-02-2017. All the pregnant ladies of different parity with USG findings of >13- weeks of gestation were included in this study. They were divided into two groups. Group-A were those with pre-eclampsia and Group-B, who were normotensive as control group. Blood samples were collected from all the participants at 15-20 weeks of gestation during antenatal visits. The serum was separated by 2,500-g centrifugation for 10-min. Aliquots of serum were stored at -80o C.

Results: In this study 384 women with pre-eclampsia group A and 50 normotensives as control Group B were studied. Mean age of group A women was 27.46+3.91 years and mean age of group B was 28.0+4.0 years, (P= 0.362). There was no significant difference between mean gestational age of group A and group B (P=0.346). Mean of systolic BP was 147.43+10.83 mmHg and diastolic 103.15+6.39 mmHg in group A ladies. Mean of soluble fms-like tyrosine kinase-1 was significantly higher 5995.5+12838.87 pg/mL, in group A ladies, when compared to group B (P= 0.0001). There was positive correlation between systolic BP and sFlt-1, (r= 0.095), as well as between diastolic BP and sFlt-1, (r= 0.039).

Conclusion: It is concluded that sFlt-1 is an important marker for the diagnosis of pre-eclampsia, as it shows significant association and positive correlation with pre-eclampsia in this study.